Journal: Brazilian Dental Journal
Article Title: Evaluation of Two Anorganic Bovine Xenogenous Grafts in Bone Healing of Critical Defect in Rats Calvaria
doi: 10.1590/0103-6440202406119
Figure Lengend Snippet: Scatter plot graphs with columns representing the immunohistochemical results for BMP2/4 (A), OCN (B) and TRAP (C). Statistical analysis of the immunostaining pattern by scores for BMP2/4, OCN and TRAP-positive cells per mm 2 in bone defects in Control, BO and BF at 30 and 45 days postoperatively. Symbols: †, a statistically significant difference in relation to control group at 30 postoperative days; ‡, a statistically significant difference in relation to control group at 45 postoperative days; ¶, a statistically significant difference in relation to the BO group at 30 postoperative days; §, a statistically significant difference in relation to the BO group at 45 postoperative days. Statistical tests for BMP2/4 and OCN: Kruskal Wallis Analysis of Variance; Student Newman-Keuls post-test; p<0.05. Statistical test for TRAP: ANOVA of Variance; Tukey post-test; p<0.05.
Article Snippet: For immunohistochemical reactions, the slides were treated by indirect immunoperoxidase technique employing the primary polyclonal antibodies to bone morphogenetic protein 2/4 (1:100, anti-BMP2/4, sc137087, Santa Cruz Biotechnology, Santa Cruz, CA), osteocalcin (1:100, anti-OCN, sc365797, Santa Cruz Biotechnology, Santa Cruz, CA) and tartrate-resistant acid phosphatase (1:100, anti-TRAP, sc376875, Santa Cruz Biotechnology, Santa Cruz, CA).
Techniques: Immunohistochemical staining, Immunostaining, Control